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Whispering gallery mode aptasensors for detection of blood proteins
Author(s) -
Pasquardini Laura,
Berneschi Simone,
Barucci Andrea,
Cosi Franco,
Dallapiccola Ramona,
Insinna Massimiliano,
Lunelli Lorenzo,
Conti Gualtiero Nunzi,
Pederzolli Cecilia,
Salvadori Simone,
Soria Silvia
Publication year - 2013
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200013
Subject(s) - biosensor , aptamer , whispering gallery wave , reusability , surface modification , nanotechnology , materials science , resonator , human blood , chemistry , optoelectronics , computer science , microbiology and biotechnology , biology , physiology , software , programming language
Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA‐aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)