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Heterodyne detected nonlinear optical imaging in a lock‐in free manner
Author(s) -
Slipchenko Mikhail N.,
Oglesbee Robert A.,
Zhang Delong,
Wu Wei,
Cheng JiXin
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201200005
Subject(s) - optics , lock in amplifier , amplifier , microscopy , materials science , signal (programming language) , heterodyne (poetry) , raman scattering , heterodyne detection , optoelectronics , preclinical imaging , physics , raman spectroscopy , laser , in vivo , microbiology and biotechnology , cmos , computer science , acoustics , biology , programming language
We report a compact, cost‐effective tuned amplifier for frequency‐selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock‐in detection, as demonstrated through stimulated Raman scattering imaging of live cells and tissues at the speed of 2 μsec/pixel. Application of the tuned amplifier to transient absorption microscopy is also demonstrated. The increased signal to noise ratio allowed epi‐detected in vivo imaging of myelin and blood in rat spinal cord with high spatial resolution. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)