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Non‐linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose
Author(s) -
Brackmann Christian,
Dahlberg JanOlof,
Vraihal Engin,
Lally Caitriona,
Gatenholm Paul,
Enejder Annika
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100141
Subject(s) - extracellular , cellulose , extracellular matrix , microscopy , smooth muscle , biophysics , chemistry , materials science , anatomy , biology , biochemistry , pathology , medicine , endocrinology
Abstract Non‐linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti‐Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non‐linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)