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Spatial orientation mapping of fibers using polarization‐sensitive second harmonic generation microscopy
Author(s) -
Hovhannisyan Vladimir A.,
Hu PoSheng,
Tan HsingYuan,
Chen SheanJen,
Dong ChenYuan
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100123
Subject(s) - azimuth , optics , polarization (electrochemistry) , materials science , microscopy , second harmonic generation , brewster's angle , second harmonic imaging microscopy , image resolution , laser , physics , chemistry , brewster
In this work, we present a non‐invasive approach to determine azimuth and elevation angles of collagen fibers capable of generating second harmonic signal. The azimuth angle was determined using the minimum of second harmonic generation (SHG) signal while rotating the plane of polarization of excitation light. The elevation angle was estimated from the ratio of the minimal SHG intensity to the intensity when laser polarization and fiber directions were parallel to each other using experimentally determined calibration curve. Pixel‐resolution images of collagen fiber spatial orientation in tendon from bovine leg, chicken leg, and chicken skin were acquired using our approach of SHG polarization‐resolved microscopy. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)