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Near‐infrared fluorescence imaging of mouse myocardial microvascular endothelium using Cy5.5‐lectin conjugate
Author(s) -
Nguyen Cecilia,
Bascaramurty Saro,
Kuzio Bozena,
Gregorash Lori,
Kupriyanov Valery,
Jilkina Olga
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100119
Subject(s) - lectin , conjugate , in vivo , ex vivo , endothelium , staining , fluorescence , chemistry , preclinical imaging , fluorescence microscope , pathology , microbiology and biotechnology , fluorescence lifetime imaging microscopy , biology , medicine , endocrinology , mathematical analysis , physics , mathematics , quantum mechanics
Cy5.5‐lectin, a non‐toxic conjugate, combines the benefits of near‐infrared (NIR) imaging, such as significant reduction of background fluorescence and increased tissue depth penetration, with its affinity for vascular endothelial cells. When compared to endothelial staining methods using FITC‐lectin and ICAM2 antibodies, Cy5.5‐lectin was confirmed to specifically bind endothelial cells and produce a fluorescence signal both in real‐time and post‐infusion. Ex‐vivo experiments with isolated hearts demonstrated that binding was limited to perfused areas of the myocardium. With mouse in‐vivo tail‐vein injections, other organs such as the liver, spleen, and kidney were also stained and yielded similar quality images of the heart. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)