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Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry
Author(s) -
Fadeev Victor V.,
Gorbunov Maxim Y.,
Gostev Timofey S.
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100113
Subject(s) - chlorella pyrenoidosa , photochemistry , quenching (fluorescence) , fluorescence , chlorophyll fluorescence , fluorescence spectroscopy , photosystem ii , singlet oxygen , chemistry , photosystem i , chlorophyll , biophysics , chlorella , algae , photosynthesis , botany , biology , optics , biochemistry , physics , organic chemistry , oxygen
We use an advanced fluorescence method of Nonlinear Laser Fluorimetry in combination with Fluorescence Induction and Relaxation technique to study the influence of excess‐light conditions on the physiological state of the green alga Chlorella pyrenoidosa . We demonstrate that zeaxanthin‐dependent non‐photochemical quenching leads to a significant increase in the rate constant of singlet‐singlet annihilation of chlorophyll a excited state, which suggests profound conformational changes in the light‐harvesting complexes of photosystem II. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)