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Bond‐selective imaging of deep tissue through the optical window between 1600 and 1850 nm
Author(s) -
Wang Pu,
Wang HanWei,
Sturek Michael,
Cheng JiXin
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100102
Subject(s) - overtone , absorption (acoustics) , excitation , chemistry , methylene blue , window (computing) , photoacoustic imaging in biomedicine , materials science , analytical chemistry (journal) , nuclear magnetic resonance , optics , physics , computer science , biochemistry , chromatography , quantum mechanics , astronomy , photocatalysis , catalysis , operating system , spectral line
We report the employment of an optical window between 1600 nm and 1850 nm for bond‐selective deep tissue imaging through harmonic vibrational excitation and acoustic detection of resultant pressure waves. In this window where a local minimum of water absorption resides, we found a 5 times enhancement of photoacoustic signal by first overtone excitation of the methylene group CH 2 at 1730 nm, compared to the second overtone excitation at 1210 nm. The enhancement allows 3D mapping of intramuscular fat with improved contrast and of lipid deposition inside an atherosclerotic artery wall in the presence of blood. Moreover, lipid and protein are differentiated based on the first overtone absorption profiles of CH 2 and methyl group CH 3 in this window. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)