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A correlative approach at characterizing nanoparticle mobility and interactions after cellular uptake
Author(s) -
Schumann Christian,
Schübbe Sabrina,
Cavelius Christian,
Kraegeloh Annette
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100064
Subject(s) - exocytosis , nanoparticle , colocalization , context (archaeology) , biophysics , endocytosis , lamellar granule , chemistry , cytoplasm , a549 cell , vesicle , intracellular , compartmentalization (fire protection) , nanotechnology , microbiology and biotechnology , cell , membrane , materials science , biology , pulmonary surfactant , biochemistry , paleontology , enzyme
The interactions of nanoparticles with human cells are of large interest in the context of nanomaterial safety. Here, we use live cell imaging and image‐based fluorescence correlation methods to determine colocalization of 88 nm and 32 nm silica nanoparticles with endocytotic vesicles derived from the cytoplasmic membrane and lysosomes, as well as to quantify intracellular mobility of internalized particles, in contrast to particle number quantification by counting techniques. In our study, A549 cells are used as a model for human type II alveolar epithelial cells. We present data supporting endocytotic uptake of the particles and subsequent active transport to the perinuclear region. The presence of particles in lamellar bodies is proposed as a potential exocytosis route. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)