Premium
Multimodal biophotonic workstation for live cell analysis
Author(s) -
Esseling Michael,
Kemper Björn,
Antkowiak Maciej,
Stevenson David J.,
Chaudet Lionel,
Neil Mark A. A.,
French Paul W.,
von Bally Gert,
Dholakia Kishan,
Denz Cornelia
Publication year - 2012
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100052
Subject(s) - workstation , chinese hamster ovary cell , microscopy , computer science , live cell imaging , digital holographic microscopy , fluorescence microscope , optical tweezers , holography , microscope , phase contrast microscopy , nanotechnology , biomedical engineering , computer graphics (images) , materials science , chemistry , fluorescence , cell , optics , physics , engineering , biochemistry , receptor , operating system
A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase‐contrast microscopy. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)