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Enhancement of imaging depth in turbid media using a wide area detector
Author(s) -
Crosignani Viera,
Dvornikov Alexander S.,
Gratton Enrico
Publication year - 2011
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201100001
Subject(s) - fluorescence , detector , optics , photon , fluorescence lifetime imaging microscopy , signal (programming language) , microscopy , two photon excitation microscopy , materials science , physics , computer science , programming language
The depth of two‐photon fluorescence imaging in turbid media can be significantly enhanced by the use of the here described fluorescence detection method that allows to efficiently collect scattered fluorescence photons from a wide area of the turbid sample. By using this detector we were able to perform imaging of turbid samples, simulating brain tissue, at depths up to 3 mm, where the two‐photon induced fluorescence signal is too weak to be detected by means used in conventional two‐photon microscopy. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)