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Fast and long term lipid droplet tracking with CARS microscopy
Author(s) -
Jüngst Christian,
Winterhalder Martin J.,
Zumbusch Andreas
Publication year - 2011
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201000120
Subject(s) - photobleaching , microscopy , fluorescence recovery after photobleaching , fluorescence microscope , term (time) , video microscopy , tracking (education) , lipid droplet , nanotechnology , chemistry , fluorescence lifetime imaging microscopy , fluorescence , biophysics , materials science , optics , physics , biology , microbiology and biotechnology , biochemistry , psychology , pedagogy , quantum mechanics
Photobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label‐free imaging techniques therefore offer advantages both for rapid image acquisition and for long–term observations. CARS microscopy is a label‐free imaging technique offering molecule specific contrast. Here we demonstrate that CARS microscopy allows video‐rate tracking of intracellular transport of lipid droplets, but also continuous long‐term observation of cells over several hours. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)