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Multimicroscopic study of curcumin effect on fixed nonmalignant and cancerous mammalian epithelial cells
Author(s) -
Saab Mariebelle,
Estephan Elias,
Bec Nicole,
Larroque Marion,
Aulombard Roger,
Cloitre Thierry,
Gergely Csilla
Publication year - 2011
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201000119
Subject(s) - curcumin , fluorescence microscope , biophysics , microtubule , fluorescence , chemistry , microscopy , microbiology and biotechnology , interphase , confocal microscopy , nanotechnology , materials science , pathology , biology , biochemistry , medicine , optics , physics
The morphology changes, in particular the organization of microtubules in mammalian nonmalignant HMEC 184A1 and cancerous MCF‐7 cells during curcumin treatment have been investigated by utilizing multiphoton, fluorescence, and atomic force (AFM) microscopies. Fluorescence microscopy reveals formation of ring‐like structures of microtubules circumscribing the nuclear area in HMEC 184A1 cells after treatment, while in MCF‐7 cells, no important changes were observed. Topography analyses of fixed HMEC 184A1 and MCF‐7 before and after treatment with curcumin were performed using AFM and the effect of the employed cells' fixation method was investigated on MCF‐7 cells. Due to its indepth optical sectioning capacity multiphoton microscopy provided valuable complementary information on curcumin's effect on both cells' types. Combining information provided by AFM and optical fluorescence and biphoton microscopes allows us to gain a better understanding of the cells and their curcumin‐induced changes, especially for microtubules which are the main target of antitumor chemotherapy treatments. Our multimicroscopic study demonstrates that 6 h incubation with curcumin does not induce significant modifications in the interphase microtubules in the malignant MCF7cell, whereas it has measurable effects on those of the nonmalignant HMEC 184A1 cells, revealing also morphology modifications over the nuclear area of these cells. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)