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Multiphoton microscopy system with a compact fiber‐based femtosecond‐pulse laser and handheld probe
Author(s) -
Liu Gangjun,
Kieu Khanh,
Wise Frank W.,
Chen Zhongping
Publication year - 2011
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201000049
Subject(s) - femtosecond , materials science , laser , optics , optoelectronics , microscopy , mobile device , pulse (music) , physics , computer science , detector , operating system
We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two‐photon signal. The hand‐held probe included galvanometer‐based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two‐photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)