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Application of Terahertz Time‐Domain Spectroscopy in Intracellular Metabolite Detection
Author(s) -
Wang Changlei,
Gong Jixian,
Xing Qirong,
Li Yanfeng,
Liu Feng,
Zhao Xueming,
Chai Lu,
Wang Chingyue,
Zheltikov Aleksei M.
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201000043
Subject(s) - riboflavin , metabolite , terahertz radiation , astaxanthin , terahertz time domain spectroscopy , intracellular , spectroscopy , terahertz spectroscopy and technology , chemistry , analytical chemistry (journal) , materials science , chromatography , biochemistry , optoelectronics , carotenoid , physics , quantum mechanics
Using terahertz time‐domain spectroscopy (THz‐TDS), we have investigated the THz spectra of astaxanthin and riboflavin and the spectra of two kinds of cell, haenatcoccus plusivalis and bacillus subtilis, which could produce astaxanthin and riboflavin, respectively, during their metabolite process. Riboflavin was found to be much more absorptive to THz radiation and have richer spectral characteristics than astaxanthin. As an intracellular metabolite, riboflavin could be distinguished from the cells by using THz‐TDS. The technique has potential applications in high‐throughput screening of industrial strains. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)