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Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy
Author(s) -
Kemper Björn,
Langehanenberg Patrik,
Höink Alexander,
von Bally Gert,
Wottowah Falk,
Schinkinger Stefan,
Guck Jochen,
Käs Josef,
Bredebusch Ilona,
Schnekenburger Jürgen,
Schütze Karin
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.201000035
Subject(s) - digital holographic microscopy , holography , microscopy , laser , microfluidics , phase contrast microscopy , materials science , optics , digital holography , focus (optics) , resolution (logic) , nanotechnology , computer science , physics , artificial intelligence
For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microfluidics to observe optically trapped living cells in a minimally invasive fashion during laser micromanipulation. The obtained results demonstrate that DHM multi‐focus phase contrast provides label‐free quantitative monitoring of optical manipulation with a temporal resolution of a few milliseconds. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)