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Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy
Author(s) -
Cicchi Riccardo,
Kapsokalyvas Dimitrios,
De Giorgi Vincenzo,
Maio Vincenza,
Van Wiechen Annelies,
Massi Daniela,
Lotti Torello,
Pavone Francesco S.
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910062
Subject(s) - dermis , elastin , autofluorescence , pathology , scars , ex vivo , connective tissue , biomedical engineering , collagen fibres , in vivo , materials science , anatomy , medicine , biology , optics , physics , microbiology and biotechnology , fluorescence
We have used nonlinear imaging to evaluate collagen organization in connective tissue ex‐vivo samples. Image analysis methods were tested on healthy dermis, normal scars, and keloids. The evaluation of the second harmonic to autofluorescence aging index of dermis (SAAID) has allowed a first characterization of tissues by scoring the collagen/elastin content. Further analyses on collagen morphology in healthy dermis and keloids were performed by image‐pattern analysis of SHG images. The gray‐level co‐occurrence matrix (GLCM) analysis method has allowed classification of different tissues based on the evaluation of geometrical arrangement of collagen fibrillar bundles, whereas a pattern analysis of the FFT images has allowed the discrimination of different tissues based on the anisotropy of collagen fibers distribution. This multiple scoring method represents a promising tool to be extended to other collagen disorders, as well as to be used in in‐vivo skin‐imaging applications. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)