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Quantified characterization of human cutaneous normal scar using multiphoton microscopy
Author(s) -
Zhu Xiaoqin,
Zhuo Shuangmu,
Zheng Liqin,
Lu Kecheng,
Jiang Xingshan,
Chen Jianxin,
Lin Bifang
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910058
Subject(s) - multiphoton fluorescence microscope , human skin , microscopy , scar tissue , epidermis (zoology) , fluorescence , autofluorescence , biomedical engineering , chemistry , fluorescence microscope , materials science , pathology , optics , anatomy , medicine , biology , surgery , physics , genetics
The morphological alterations of human cutaneous normal scar were quantitatively analyzed using multiphoton microscopy (MPM) based on two‐photon excited fluorescence and second harmonic generation. High‐contrast, high‐resolution images of normal scar and uninjured skin were obtained for comparison. In addition, some quantitative parameters have been extracted to quantitatively discriminate between normal scar and uninjured skin. The MPM combined with quantitative method enable a better understanding of microstructual alterations of the epidermis, elastic fiber, and collagen in normal scar. It may lead the way to making know the mechanism of normal scar formation and identifying feasible therapeutic options. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)