Premium
In vivo fiber‐based multicolor photoacoustic detection and photothermal purging of metastasis in sentinel lymph nodes targeted by nanoparticles
Author(s) -
Galanzha Ekaterina I.,
Kokoska Mimi S.,
Shashkov Evgeny V.,
Kim JinWoo,
Tuchin Valery V.,
Zharov Vladimir P.
Publication year - 2009
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910046
Subject(s) - lymphatic system , sentinel lymph node , photothermal therapy , lymph , metastasis , flow cytometry , medicine , in vivo , pathology , micrometastasis , melanoma , biomedical engineering , ex vivo , cancer , breast cancer , cancer research , materials science , biology , immunology , nanotechnology , microbiology and biotechnology
This report introduces a novel diagnostic and therapeutic platform for in vivo non‐invasive detection and treatment of metastases in sentinel lymph nodes (SLNs) at single cell level using an integrated system of multicolor photoacoustic (PA) lymph flow cytometry, PA lymphography, absorption image cytometry, and photothermal (PT) therapy. A melanoma‐bearing mouse model was used to demonstrate the capability of this platform for real‐time lymphatic mapping, counting of disseminated tumor cells (DTCs) in prenodal lymphatics, and detecting metastasis in SLNs and its purging. The detection and ablation of non‐pigmented breast cancer cells in SLNs was achieved by labeling them with nanoparticles. The association between DTC count and SLN metastasis progression supports lymphatic DTCs as a novel prognostic marker of metastasis. The fiber‐based portable PA device may replace the conventional SLN(s) excision and histology‐based staging. The earliest detection of DTCs in the lymphatic vessels before the establishment of nodal metastasis may prevent metastasis by well‐timed ablation of DTCs. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)