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Flow cytometry with gold nanoparticles and their clusters as scattering contrast agents: FDTD simulation of light–cell interaction
Author(s) -
Tanev Stoyan,
Sun Wenbo,
Pond James,
Tuchin Valery V.,
Zharov Vladimir P.
Publication year - 2009
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910039
Subject(s) - finite difference time domain method , flow cytometry , contrast (vision) , nanoparticle , light scattering , colloidal gold , scattering , flow (mathematics) , materials science , nanotechnology , optics , chemistry , physics , mechanics , medicine , immunology
The formulation of the finite‐difference time‐domain (FDTD) approach is presented in the framework of its potential applications to in‐vivo flow cytometry based on light scattering. The consideration is focused on comparison of light scattering by a single biological cell alone in controlled refractive‐index matching conditions and by cells labeled by gold nanoparticles. The optical schematics including phase contrast (OPCM) microscopy as a prospective modality for in‐vivo flow cytometry is also analyzed. The validation of the FDTD approach for the simulation of flow cytometry may open up a new avenue in the development of advanced cytometric techniques based on scattering effects from nanoscale targets. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)