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Concurrent observations of astrocytic Ca 2+ activity and multisite extracellular potentials from an intact cerebral cortex
Author(s) -
Riera Jorge,
Ogawa Takeshi,
Hatanaka Rieko,
Goto Takakuni,
Sumiyoshi Akira,
Kadji Herve Enjieu,
Nakauchi Sakura,
Kawashima Ryuta
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910036
Subject(s) - extracellular , neuroscience , astrocyte , local field potential , premovement neuronal activity , biophysics , chemistry , physics , computer science , biology , microbiology and biotechnology , central nervous system
In basic neuroscience, the attention has been recently focused on the role played by the protoplasmic astrocytes in modulating the activity of nearby neurons or else on assisting a long‐term/sustained communication between these neurons and the surrounding microvasculature. However, to understand the physiological mechanisms underlying such a multiscale interactions in space and time, novel methodologies are required. This paper reports about an experimental setting and a procedure that was developed to obtain concurrently two‐photon astrocytic Ca 2+ imaging and multisite large‐scale extracellular potentials as recorded by a silicon‐based probe. Solutions to several technical drawbacks (e.g. removal of photoelectric artifacts, the establishment of safety ranges for microinjection) are provided which are intrinsic to the technology and procedure utilized. Through the use of SR101 to stain protoplasmic astrocytes, it was possible to combine functional information represented by the Ca 2+ activity in individual astrocytes and the LFPs with geometrical descriptors of the astrocytic/vessel networks. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)