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Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart
Author(s) -
Bradu Adrian,
Ma Lisha,
Bloor James W.,
Podoleanu Adrian
Publication year - 2009
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910021
Subject(s) - optical coherence tomography , drosophila melanogaster , fluorescence microscope , microscopy , biomedical engineering , fluorescence , fluorescence lifetime imaging microscopy , optics , medicine , biology , radiology , pathology , physics , biochemistry , gene
This article demonstrates a combined instrument of two imaging modalities to acquire information on cardiac function in larval Drosophila melanogaster : optical coherence tomography (OCT) and laser scanning fluorescence microscopy (LSFM). For this purpose, a dedicated imaging instrument able to sequentially provide cross‐sectional OCT and C‐scan LSFM images has been developed. With this dual‐imaging system, the heart can be easily located and visualized within the specimen and the change of the heart shape in a cardiac cycle can be monitored. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)