Premium Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamersPremium
Author(s)
Hernandez Frank Jeyson,
Dondapati Srujan Kumar,
Ozalp V. Cengiz,
Pinto Alessandro,
O'Sullivan Ciara K.,
Klar Thomas A.,
Katakis Ioannis
Publication year2009
Publication title
journal of biophotonics
Resource typeJournals
PublisherWILEY‐VCH Verlag
Abstract Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta‐nano‐sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta‐sensor may be used in nanoscopic volumes such as in array type assays or even inside cells. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Subject(s)aptamer , avidin , biochemistry , biology , biosensor , biotin , chemistry , colloidal gold , materials science , metal , metallurgy , microbiology and biotechnology , nanoparticle , nanoscopic scale , nanotechnology , noble metal
Language(s)English
SCImago Journal Rank0.877
H-Index66
eISSN1864-0648
pISSN1864-063X
DOI10.1002/jbio.200910006
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