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Label free optical sensor for Avidin based on single gold nanoparticles functionalized with aptamers
Author(s) -
Hernandez Frank Jeyson,
Dondapati Srujan Kumar,
Ozalp V. Cengiz,
Pinto Alessandro,
O'Sullivan Ciara K.,
Klar Thomas A.,
Katakis Ioannis
Publication year - 2009
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910006
Subject(s) - aptamer , nanotechnology , nanoscopic scale , biosensor , nanoparticle , colloidal gold , avidin , noble metal , materials science , chemistry , metal , biotin , microbiology and biotechnology , biochemistry , biology , metallurgy
Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta‐nano‐sensor is in the range of 20 nM. Due to its nanoscopic size, this single nanoparticle based apta‐sensor may be used in nanoscopic volumes such as in array type assays or even inside cells. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)