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Selective targeting of green fluorescent nanodiamond conjugates to mitochondria in HeLa cells
Author(s) -
Mkandawire Msaukiranji,
Pohl Andrea,
Gubarevich Tatiana,
Lapina Victoria,
Appelhans Dietmar,
Rödel Gerhard,
Pompe Wolfgang,
Schreiber Jürgen,
Opitz Jörg
Publication year - 2009
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200910002
Subject(s) - nanodiamond , hela , intracellular , fluorescence microscope , transfection , fluorescence , autofluorescence , liposome , chemistry , biophysics , microbiology and biotechnology , nanotechnology , cell , biochemistry , biology , materials science , diamond , physics , organic chemistry , quantum mechanics , gene
Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Transfections were mediated by 4 th ‐generation dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful transfections of the nanodiamonds into HeLa cells. Nanodiamond fluorescence could be easily differentiated from cellular autofluorescence. Furthermore, nanodiamonds could be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for intracellular assays. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)