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Towards an optical biopsy for the diagnosis of breast cancer in vivo by endogenous fluorescence spectroscopy
Author(s) -
Alchab Lama,
Dupuis Guillaume,
Balleyguier Corinne,
Mathieu MarieChristine,
FontaineAupart MariePierre,
Farcy René
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200900070
Subject(s) - in vivo , breast cancer , fluorescence , confocal , biopsy , pathology , confocal microscopy , medicine , endogeny , autofluorescence , fluorescence spectroscopy , fluorescence microscope , cancer , radiology , biomedical engineering , chemistry , optics , biology , physics , microbiology and biotechnology
The techniques of medical imaging allow the detection of suspect lesions in the breast, but they do not always evidence the malignant nature of these lesions. Breast biopsies and histological analyses are therefore implemented to establish a diagnosis. In order to reduce the number of these invasive procedures, a portable clinical system was designed based upon the excitation of Endogenous Fluorescence in vivo at 405 nm via a fiber‐optics probe included in a disposable needle of small diameter (<1 mm). From the fluorescence signal, the authors are able to discriminate between diseased and healthy areas of human breast biopsies. Stronger fluorescence intensity and systematic spectral red shift of the tumor areas were observed. These results are confirmed by confocal microscopy. This new instrument is promising for the minimally invasive diagnosis of breast tumors in vivo with an appreciable limitation of patient trauma and of operational and financial cost. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)