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Interpreting diffuse reflectance for in vivo skin reactions in terms of chromophores
Author(s) -
Kollias Nikiforos,
Seo InSeok,
Bargo Paulo R.
Publication year - 2010
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200900066
Subject(s) - chromophore , absorbance , superposition principle , chemistry , linear relationship , photochemistry , human skin , biophysics , optics , biological system , chromatography , physics , mathematics , mathematical analysis , biology , statistics , genetics
Abstract The measurement and quantification of skin reactions to insults involves certain assumptions about the relation between intensity of color appearance of the skin and the concentration of endogenous chromophores. The underlying assumption is that the Beer‐Lambert law is obeyed, i.e., that a linear relation exists between the absorbance and the concentration of each chromophore and that the total absorbance is the linear superposition of the contributions of each chromophore. In this paper the authors compiled the results from a number of interventions on human skin that result in changes in its appearance and small deviations from the homeostatic state, where the results may be accounted for by a single or multiple chromophores. The validity of the assumptions is found to hold for a limited range of responses. The biological constraints need to be considered in certain cases because as we move away from the homeostatic state, complex biological processes are induced. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)