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A compact, multidimensional spectrofluorometer exploiting supercontinuum generation
Author(s) -
Manning Hugh B.,
Kennedy Gordon T.,
Owen Dylan M.,
Grant David M.,
Magee Anthony I.,
Neil Mark A. A.,
Itoh Yoshifumi,
Dunsby Chris,
French Paul M.W.
Publication year - 2008
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200810051
Subject(s) - supercontinuum , förster resonance energy transfer , autofluorescence , laser , ultrashort pulse , optics , materials science , wavelength , optoelectronics , fluorescence , physics , photonic crystal fiber
We report a novel, compact and automated multidimensional spectrofluorometer that exploits a fibre‐laser‐pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase‐sensitive membrane probe di‐4‐ANEPPDHQ and to characterise protein‐protein interactions via Förster resonance energy transfer. It can be applied to in situ measurements via a fibre‐optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)