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Assessing the risk of skin damage due to femtosecond laser irradiation
Author(s) -
Fischer Frank,
Volkmer Beate,
Puschmann Stefan,
Greinert Rüdiger,
Breitbart Eckhard,
Kiefer Jürgen,
Wepf Roger
Publication year - 2008
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200810050
Subject(s) - femtosecond , irradiation , laser , materials science , medicine , optics , physics , nuclear physics
Abstract We irradiated freshly excised skin biopsies with four irradiation regimes usually taken for multiphoton imaging. If there is any skin damaging, it is mainly an effect similar to the damaging effects of UV‐irradiation. Using fluorescent antibodies against cyclobutane‐pyrimidin‐dimers (CPDs) in combination with immuno‐fluorescence image analysis we quantitatively compared fs‐irradiation effects with UV‐irradiation (solar simulator). Based on these results we are giving a risk assessment. The results show that multi photon imaging using the parameters described here is in the ballpark of damaging occurring from every day sun exposure. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)