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Multiphoton tissue imaging using high‐NA microendoscopes and flexible scan heads for clinical studies and small animal research
Author(s) -
König Karsten,
Weinigel Martin,
Hoppert Daniel,
Bückle Reiner,
Schubert Harald,
Köhler Martin J.,
Kaatz Martin,
Elsner Peter
Publication year - 2008
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200810049
Subject(s) - optics , lens (geology) , femtosecond , autofluorescence , gradient index optics , numerical aperture , two photon excitation microscopy , materials science , biomedical engineering , elastin , photodetector , preclinical imaging , laser , in vivo , physics , refractive index , medicine , fluorescence , pathology , wavelength , microbiology and biotechnology , biology
Multiphoton tomographs based on femtosecond laser and GRIN lens technology in combination with flexible scan heads have been developed for clinical high‐resolution tissue imaging and small animal research. The novel tissue tomograph possesses a 0.5 m long flexible mirror arm in combination with piezodriven focusing optics and multiple single photon counting PMT detectors. The photodetectors are in particular useful to obtain information on the extracellular matrix by the simultaneous measurement of the two‐photon autofluorescence of elastin as well as the second harmonic generation of collagen. A major application is the in vivo determination of the skin age index. The rigid two‐photon microendoscope with a high numerical aperture of 0.8 is based on a combination of silver‐doped 1.0 mm rod‐shaped GRIN lens with a hemispheric front optics. It was used in combination with the multiphoton tomograph for clinical studies as well as for inner organ and eye imaging of small animals. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)