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Structural stability of green fluorescent proteins entrapped in polyelectrolyte nanocapsules
Author(s) -
Krol Silke,
Can Fabio,
Caccia Michele,
Sironi Laura,
Bianchini Paolo,
Campanini Barbara,
Collini Maddalena,
Chirico Giuseppe,
Diaspro Alberto
Publication year - 2008
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200810033
Subject(s) - polyelectrolyte , nanocapsules , chemistry , fluorescence , biophysics , denaturation (fissile materials) , polymer , chemical modification , molecule , chemical engineering , nanotechnology , polymer chemistry , organic chemistry , nanoparticle , materials science , nuclear chemistry , physics , quantum mechanics , engineering , biology
Molecules of a green fluorescent protein mutant, GFPmut2, have been immobilized in nanocapsules, assemblies of charged polyelectrolyte multilayers, with the aim to study the effect of protein‐polyelectrolyte interactions on the protein stability against chemical denaturation. GFPmut2 proteins turn out to be stabilized and protected against the denaturating action of small chemical compounds. The nanocapsule protective effect on GFPmut2 is likely due to protein interactions with the negatively charged polymers, that induce an increase in the local rigidity of the protein nano‐environment. This suggestion is supported by Fluorescence Polarization measurements on GFPmut2 proteins bound to the NC layers. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)