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Spatial fluorescence cross‐correlation spectroscopy by means of a spatial light modulator
Author(s) -
Blancquaert Yoann,
Gao Jie,
Derouard Jacques,
Delon Antoine
Publication year - 2008
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200810007
Subject(s) - fluorescence correlation spectroscopy , fluorescence , spectroscopy , fluorescence cross correlation spectroscopy , fluorescence spectroscopy , spatial light modulator , rhodamine , laser induced fluorescence , spatial correlation , analytical chemistry (journal) , chemistry , materials science , optics , physics , optoelectronics , chromatography , computer science , telecommunications , quantum mechanics
Spatial fluorescence cross‐correlation spectroscopy is a rarely investigated version of fluorescence correlation spectroscopy, in which the fluorescence signals from different observation volumes are cross‐correlated. In the reported experiments, two observation volumes, typically shifted by a few μm, are produced, with a spatial light modulator and two adjustable pinholes. We illustrated the feasibility and potentiality of this technique by: i) measuring molecular flows, in the range 0.2–1.5 μm/ms, of solutions seeded with fluorescent nanobeads or rhodamine molecules (simulating active transport phenomenons); ii) investigating the permeability of the phospholipidic membrane of giant unilamellar vesicles versus hydrophilic or hydrophobic molecules (in that case the laser spots were set on both sides of the membrane). Theoretical descriptions are proposed together with a discussion about fluorescence‐correlation‐spectroscopy‐based, alternative methods. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)