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Nonlinear laser imaging of skin lesions
Author(s) -
Cicchi R.,
Sestini S.,
De Giorgi V.,
Massi D.,
Lotti T.,
Pavone F. S.
Publication year - 2008
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.200710003
Subject(s) - microscopy , human skin , basal cell carcinoma , two photon excitation microscopy , pathology , ex vivo , fluorescence lifetime imaging microscopy , keloid , fluorescence microscope , in vivo , fluorescence , skin cancer , multispectral image , materials science , second harmonic generation , multiphoton fluorescence microscope , laser , medicine , basal cell , optics , cancer , biology , genetics , physics , microbiology and biotechnology , computer science , computer vision
We investigated different kinds of human ex‐vivo skin samples by combined two‐photon intrinsic fluorescence (TPE), second‐harmonic generation microscopy (SHG), fluorescence lifetime imaging microscopy (FLIM), and multispectral two‐photon emission detection (MTPE). Morphological and spectroscopic differences were found between healthy and pathological skin samples, including tumors. In particular, we examined tissue samples from normal and pathological scar tissue (keloid), and skin tumors, including basal cell carcinoma (BCC), and malignant melanoma (MM). By using combined TPE‐SHG microscopy we investigated morphological features of different skin regions. Further comparative analysis of healthy skin and neoplastic samples was performed using FLIM, and MTPE. Finally, we demonstrated the use of methyl‐aminolevulinate as a contrast agent to increase the contrast in BCC border detection. The results obtained represent further support for in‐vivo noninvasive imaging of diseased skin. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)