Premium
Strain‐ and sex‐related differences of carbonyl reductase activities in kidney microsomes and cytosol of rats
Author(s) -
Imamura Yorishige,
Shimada Hideaki
Publication year - 2004
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.996
Subject(s) - microsome , kidney , medicine , enzyme , endocrinology , cytosol , strain (injury) , reductase , metabolism , chemistry , biochemistry , biology
This study was designed to elucidate strain‐ and sex‐related differences of carbonyl reductase activity in rat kidney by using the oral antidiabetic drug acetohexamide as substrate. The frequency distribution of carbonyl reductase activities in kidney microsomes of male Fischer 344 (Fischer), Sprague‐Dawley, Wistar and Wistar‐Imamichi (Wistar‐IM) rats exhibited a marked strain‐related difference. Furthermore, the enzyme activities in kidney microsomes of Fischer, Sprague‐Dawley and Wistar rats were male‐specic, resulting in signicant sex‐related differences in these strains. There was no sex‐related difference of carbonyl reductase activity in kidney microsomes of the Wistar‐IM strain, which lacked its activity in both sexes. On the other hand, although carbonyl reductase activities were fully detectable in kidney cytosols from all the strains of male and female rats, no strain‐ or sex‐related difference was observed among the cytosolic enzyme activities. These results provide new information for understanding the inuence of internal factors on the renal metabolism of ketone‐containing xenobiotics. Copyright © 2004 John Wiley & Sons, Ltd.