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Increased hepatic acylcarnitines after oral administration of amiodarone in rats
Author(s) -
Kim Gabin,
Choi HyungKyoon,
Lee Hwanhui,
Moon KyoungSik,
Oh Jung Hwa,
Lee Jaeick,
Shin Jae Gook,
Kim Dong Hyun
Publication year - 2020
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3960
Subject(s) - amiodarone , endocrinology , medicine , carnitine , metabolism , oral administration , lipid metabolism , liver injury , drug metabolism , endogeny , chemistry , biology , atrial fibrillation
Amiodarone is known to induce hepatic injury in some recipients. We applied an untargeted metabolomics approach to identify endogenous metabolites with potential as biomarkers for amiodarone‐induced liver injury. Oral amiodarone administration for 1 week in rats resulted in significant elevation of acylcarnitines and phospholipids in the liver. Hepatic short‐ and medium‐chain acylcarnitines were dramatically increased in a dose‐dependent manner, while the serum levels of these acylcarnitines did not change substantially. In addition, glucose levels were significantly increased in both the serum and liver. Gene expression profiling showed that the hepatic mRNA levels of Cpt1 , Cpt2 , and Acat1 were significantly suppressed, whereas those of Acot1 , Acly , Acss2 , and Acsl3 were increased. These results suggest that hepatic acylcarnitines and glucose levels might be increased due to disruption of mitochondrial function and suppression of glucose metabolism. Perturbation of energy metabolism might be associated with amiodarone‐induced hepatotoxicity.