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A prospective pilot study of the T‐lymphocyte response to fine particulate matter exposure
Author(s) -
Al Zallouha Margueritta,
Landkocz Yann,
Méausoone Clémence,
Ledoux Fréderic,
Visade Fabien,
Cazier Fabrice,
Martin Perrine J.,
Borgie Mireille,
Vitagliano JeanJacques,
Trémolet Gauthier,
Cailliez JeanCharles,
Gosset Pierre,
Courcot Dominique,
Billet Sylvain
Publication year - 2020
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3932
Subject(s) - immune system , ex vivo , transcriptome , cyp1b1 , cytokine , lymphocyte , physiology , immunology , peripheral blood mononuclear cell , in vivo , toxicity , biology , medicine , gene expression , in vitro , gene , metabolism , biochemistry , cytochrome p450 , microbiology and biotechnology
Exposure to air pollution is associated with increased morbidity and mortality. Once the fine atmospheric particulate matter (FP) is inhaled, some of its compounds can pass through the lungs and reach the bloodstream where they can come into contact with immune cells. Exposure to FP particularly affects sensitive populations such as the elderly. Aging affects the immune system, making the elderly more vulnerable. The project aims to determine the effects of FP exposure on human T cells while looking for biomarkers associated with exposure. Blood samples from 95 healthy subjects in three different age groups (20‐30, 45‐55 and 70‐85 years) were collected to determine a potential age effect. T lymphocytes were isolated to be exposed ex vivo for 72 hours to 45 μg/mL of FP collected in Dunkirk and chemically characterized. Overexpression of the CYP1A1 , CYP1B1 and CYP2S1 genes was therefore measured after exposure of the T cells to FP. These genes code for enzymes known to be involved in the metabolic activation of organic compounds such as polycyclic aromatic hydrocarbons detected in the FP sample. T‐cell profiling allowed us to suggest a mixed T‐helper 1/2 profile caused by exposure to FP. With regard to the influence of age, we have observed differences in the expression of certain genes, as well as an increase in interleukin‐4 and ‐13 concentrations in the elderly. These results showed that exposure of T lymphocytes to FP causes effects on both transcriptomic and cytokine secretion levels.