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Comparison of microRNA expressions for the identification of chemical hazards in in vivo and in vitro hepatic injury models
Author(s) -
Hwang SoRyeon,
Tham Nga Thi Thu,
Lee Sooho,
Bang JiHyun,
Yi Hee,
Park YoungIl,
Lee HyunKyoung,
Kang HwanGoo,
Kim YongSang,
Woo GyeHyeong,
Ku HyunOk
Publication year - 2019
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3722
Subject(s) - in vivo , microrna , in vitro , liver injury , acetaminophen , in vitro toxicology , pharmacology , downregulation and upregulation , biology , chemistry , biochemistry , gene , microbiology and biotechnology
Biofluid‐based biomarkers provide an efficient tool for hazard identification of chemicals. Here, we explored the potential of microRNAs (miRNAs) as biomarkers for hepatotoxicity of chemicals by linking in vitro to in vivo animal models. A search of the literature identified candidate circulating miRNA biomarkers of chemical‐induced hepatotoxicity. The expression of candidate miRNAs (miR‐122, miR‐151a, miR‐192, miR‐193a, miR‐194, miR‐21, miR‐29c), was determined by real‐time reverse transcription‐polymerase chain reaction in in vivo acute liver injury induced by acetaminophen, and then were further compared with those of in vitro cell assays. Candidate miRNAs, except miR‐29c, were significantly or biologically upregulated by acetaminophen, at a dose that caused acute liver injury as confirmed by hepatocellular necrosis. Except miR‐122 and miR‐193a, other miRNAs elevated in in vivo models were confirmed by in vitro models using HepG2 cells, whereas they failed by in vitro models using human primary hepatocytes. These findings indicate that certain miRNAs may still have the potential of toxicological biomarkers in linking in vitro to in vivo hepatotoxicity.