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A genomic characterization of the influence of silver nanoparticles on bone differentiation in MC3T3‐E1 cells
Author(s) -
Qing Tao,
Mahmood Meena,
Zheng Yuanting,
Biris Alexandru S.,
Shi Leming,
Casciano Daniel A.
Publication year - 2018
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3528
Subject(s) - silver nanoparticle , osteoclast , microbiology and biotechnology , bone remodeling , chemistry , morphogenesis , osteoblast , cellular differentiation , in vitro , gene expression , microrna , biology , gene , nanoparticle , endocrinology , nanotechnology , biochemistry , materials science
Silver nanoparticles (AgNPs) have been widely used in a variety of biomedical applications. Previous studies demonstrated that AgNPs significantly enhanced bone cell mineralization and differentiation in MC3T3–1 cells, a model in vitro system, when compared to several other NPs. This increased bone deposition was evaluated by phenotypic measurements and assessment of the expression of miRNAs associated with regulation of bone morphogenic proteins. In the present study, we used RNA‐seq technology, a more direct measurement of gene expression, to investigate further the mechanisms of bone differentiation induced by AgNP treatment. Key factors associated with the osteoclast pathway were significantly increased in response to AgNP exposure including Bmp4, Bmp6 and Fosl1. In addition, genes of metabolism and toxicity pathways were significantly regulated as well. Although this study suggests the potential for AgNPs to influence bone morphogenesis in injury or disease applications, further investigation into the efficacy and safety of AgNPs in bone regeneration is warranted.