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AMPK activator acadesine fails to alleviate isoniazid‐caused mitochondrial instability in HepG2 cells
Author(s) -
Zhang TianGuang,
Ikejima Takashi,
Hayashi Toshihiko,
Zhao Jun,
Wang YiMei,
Peng ShuangQing
Publication year - 2017
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3483
Subject(s) - ampk , activator (genetics) , isoniazid , pharmacology , mitochondrion , medicine , chemistry , tuberculosis , phosphorylation , biochemistry , protein kinase a , pathology , receptor
Isoniazid (INH) is a first‐line antituberculosis drug that is adversely associated with hepatotoxicity. Recently, impairment of mitochondrial homeostasis involved in this side effect has been noticed. Mitochondrial homeostasis is achieved by the balance between the generation of functional mitochondria by biogenesis and elimination of dysfunctional mitochondria by autophagy. AMP‐activated protein kinase (AMPK) can maintain mitochondrial stability through positive control of these two processes. In this study, we showed that AMPK activator acadesine (AICAR) alleviated INH‐caused impairment of mitochondrial biogenesis by activation of silent information regulator two ortholog 1 (SIRT1)–peroxisome proliferator‐activated receptor γ coactivator 1α (PGC1 α) pathway in HepG2 cells. However, mitochondrial instability and apoptosis were caused by AICAR along with an unexpected decrease in INH‐induced cytoprotective autophagy. Therefore, AICAR failed to alleviate INH‐caused mitochondrial instability in HepG2 cells due to its inhibitory effect on autophagy induced by INH. Copyright © 2017 John Wiley & Sons, Ltd.