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Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna , and may thus act as a target for chemical disruption of molting
Author(s) -
Sumiya Eri,
Ogino Yukiko,
Toyota Kenji,
Miyakawa Hitoshi,
Miyagawa Shinichi,
Iguchi Taisen
Publication year - 2016
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3306
Subject(s) - ecdysteroid , biology , gene knockdown , embryogenesis , moulting , ecdysone , daphnia magna , embryo , microbiology and biotechnology , embryonic stem cell , medicine , endocrinology , genetics , gene , hormone , botany , toxicity , larva
To understand the physiological roles of ecdysteroids in Daphnia magna embryos, we analyzed the genes for ecdysteroid synthesis or degradation. The innate ecdysteroids titer during the embryogenesis showed two peaks after which embryonic moltings occur. Expression of neverland1 and shade increased before the first peak. Knocking‐down neverland1 or shade inhibited embryonic moltings. Neverland1 ‐knowckdown embryos showed lowered ecdysteroids titer. In situ hybridization revealed neverland1 was expressed in embryonic gut epithelium suggesting the site for an initial step of ecdysteroid synthesis. Copyright © 2016 John Wiley & Sons, Ltd.