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An assay to determine the sensitive window of embryos to chemical exposure using Xenopus tropicalis
Author(s) -
Hu Lingling,
Wu Lijiao,
Xue Yingang,
Zhu Jingmin,
Shi Huahong
Publication year - 2016
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3200
Subject(s) - xenopus , embryo , retinoic acid , teratology , biology , developmental toxicity , toxicity , andrology , toxicology , chemical compound , chemistry , biochemistry , genetics , cell culture , medicine , gene , pregnancy , gestation , organic chemistry
The frog embryo teratogenesis assay‐ Xenopus (FETAX) is an established method to evaluate the developmental toxicity of chemicals. In FETAX, a 48 h continuous exposure is usually conducted when the X . tropicalis embryo is used as the test model. In the present study, we exposed X . tropicalis embryos to nine known teratogens for four separate 12‐h periods. The embryos showed great variations in response to nine tested compounds during different exposure periods. Based on the value of the score of malformations, the most sensitive 12 h exposure periods of embryos were significantly distinguished for all the compounds with the exception of NiCl 2 . The embryos were the most sensitive to retinols (e.g. all‐trans ‐retinoic acid and 9‐cis ‐retinoic acid) during 0–12 h and to metal compounds (e.g. triphenlytin and CdCl 2 ) during a 24 to 36 h exposure period. In the further 3 h exposure experiment, the most sensitive period could only be determined for one of three tested compounds. Based on the present results, we proposed an assay to determine a 12 h sensitive window of embryos to chemical exposure using Xenopus tropicalis . Copyright © 2015 John Wiley & Sons, Ltd.