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BDE‐209 inhibits pluripotent genes expression and induces apoptosis in human embryonic stem cells
Author(s) -
Du Lili,
Sun Wen,
Zhang Huili,
Chen Dunjin
Publication year - 2016
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3195
Subject(s) - homeobox protein nanog , embryonic stem cell , sox2 , downregulation and upregulation , induced pluripotent stem cell , stem cell , microbiology and biotechnology , biology , apoptosis , reactive oxygen species , andrology , genetics , medicine , gene
Decabromodiphenyl ether (BDE‐209) has been detected in human serum, semen, placenta, cord blood and milk worldwide. However, little is known regarding the potential effects on the early human embryonic development of BDE‐209. In this study, human embryonic stem cell lines FY‐hES‐10 and FY‐hES‐26 were used to evaluate the potential effects and explore the toxification mechanisms using low‐level BDE‐209 exposure. Our data showed that BDE‐209 exposure (1, 10 and 100 nM) reduced the expression of pluripotent genes such as OCT4, SOX2 and NANOG and induced human embryonic stem cells (hESCs) apoptosis. The downregulation of BIRC5/BCL2 and upregulation of BAX were related to apoptosis of hESCs induced by BDE‐209 exposure. A mechanism study showed that OCT4 down‐regulation accompanied by OCT4 promoter hypermethylation and increasing miR‐145/miR‐335 levels, OCT4 inhibitors. Moreover, BDE‐209 could increase the generation of intracellular reactive oxygen species (ROS) and decrease SOD2 expression. The ROS increase and OCT4 downregulation after BDE‐209 exposure could be reversed partly by antioxidant N‐acetylcysteine supplement. These findings showed that BDE‐209 exposure could decrease pluripotent genes expression via epigenetic regulation and induce apoptosis through ROS generation in human embryonic stem cells in vitro . Copyright © 2015 John Wiley & Sons, Ltd.