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The effect of Fe 2 O 3 and ZnO nanoparticles on cytotoxicity and glucose metabolism in lung epithelial cells
Author(s) -
Lai Xiaofeng,
Wei Yifang,
Zhao Hu,
Chen Suning,
Bu Xin,
Lu Fan,
Qu Dingding,
Yao Libo,
Zheng Jianyong,
Zhang Jian
Publication year - 2015
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3128
Subject(s) - cytotoxicity , metabolism , chemistry , nanoparticle , carbohydrate metabolism , lung , biophysics , biochemistry , nuclear chemistry , radiochemistry , nanotechnology , materials science , medicine , biology , in vitro
Metallic nanoparticles (NPs) have potential applications in industry and medicine, but they also have the potential to cause many chronic pulmonary diseases. Mechanisms for their cytotoxicity, glucose and energy metabolism responses need to be fully explained in lung epithelial cells after treatment with metallic nanoparticles. In our study, two different metallic nanoparticles (Fe 2 O 3 and ZnO) and two cell‐based assays (BEAS‐2B and A549 cell lines) were used. Our findings demonstrate that ZnO nanoparticles, but not Fe 2 O 3 nanoparticles, induce cell cycle arrest, cell apoptosis, reactive oxygen species (ROS) production, mitochondrial dysfunction and glucose metabolism perturbation, which are responsible for cytotoxicity. These results also suggest that the glucose metabolism and bioenergetics had a great potential in evaluating the cytotoxicity and thus were very helpful in understanding their underlying molecular mechanisms. Copyright © 2015 John Wiley & Sons, Ltd.