Premium
Developmental toxicity assay using high content screening of zebrafish embryos
Author(s) -
LantzMcPeak Susan,
Guo Xiaoqing,
Cuevas Elvis,
Dumas Melanie,
Newport Glenn D.,
Ali Syed F.,
Paule Merle G.,
Kanungo Jyotshna
Publication year - 2015
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.3029
Subject(s) - developmental toxicity , danio , zebrafish , high content screening , teratology , toxicity , embryo , toxicant , biology , toxicology , pharmacology , chemistry , biochemistry , microbiology and biotechnology , genetics , pregnancy , gestation , organic chemistry , gene , cell
Abstract Typically, time‐consuming standard toxicological assays using the zebrafish ( Danio rerio ) embryo model evaluate mortality and teratogenicity after exposure during the first 2 days post‐fertilization. Here we describe an automated image‐based high content screening (HCS) assay to identify the teratogenic/embryotoxic potential of compounds in zebrafish embryos in vivo . Automated image acquisition was performed using a high content microscope system. Further automated analysis of embryo length, as a statistically quantifiable endpoint of toxicity, was performed on images post‐acquisition. The biological effects of ethanol, nicotine, ketamine, caffeine, dimethyl sulfoxide and temperature on zebrafish embryos were assessed. This automated developmental toxicity assay, based on a growth‐retardation endpoint should be suitable for evaluating the effects of potential teratogens and developmental toxicants in a high throughput manner. This approach can significantly expedite the screening of potential teratogens and developmental toxicants, thereby improving the current risk assessment process by decreasing analysis time and required resources. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.