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Establishment of a short‐term, in vivo screening method for detecting chemicals with juvenile hormone activity using adult Daphnia magna
Author(s) -
Abe Ryoko,
Watanabe Haruna,
Yamamuro Masumi,
Iguchi Taisen,
Tatarazako Norihisa
Publication year - 2015
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2989
Subject(s) - daphnia magna , offspring , juvenile hormone , biology , in vivo , juvenile , endocrinology , ovary , medicine , hormone , ovulation , toxicology , toxicity , pregnancy , genetics
Juvenile hormone (JH) and JH agonists have been shown to induce male offspring production in various daphnids, including Daphnia magna using OECD TG211. The critical period (about 1h) for JH action on ova in the parent's ovary to induce male offspring is existing at 7‐8h later from ovulation. Therefore, we considered that adult D . magna could be used to produce a short‐term screening method for detecting JH analogs. Using this method, we successfully demonstrated male offspring induction in the second broods after exposure to JH or JH agonists. After investigating the exposure time, the number of repetitions and the exposure concentration, we established a short‐term, in vivo screening method for detecting JH analogs using adult D . magna . We examined positive and negative control chemicals using a previously developed method and verified the validity of our new testing method. Copyright © 2014 John Wiley & Sons, Ltd.