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Protective effect of curcumin against formaldehyde‐induced genotoxicity in A549 Cell Lines
Author(s) -
Zhang BenYan,
Shi YuQin,
Chen Xin,
Dai Juan,
Jiang ZhongFa,
Li Ning,
Zhang ZhiBing
Publication year - 2013
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2814
Subject(s) - genotoxicity , curcumin , a549 cell , chemistry , lipid peroxidation , oxidative stress , reactive oxygen species , superoxide dismutase , glutathione peroxidase , malondialdehyde , antioxidant , comet assay , glutathione , dna damage , biochemistry , pharmacology , toxicity , cell , biology , dna , enzyme , organic chemistry
Formaldehyde is ubiquitous in the environment. It is known to be a genotoxic substance. We hypothesized that reactive oxygen species (ROS) and lipid peroxidation are involved in formaldehyde‐induced genotoxicity in human lung cancer cell lines A549. To test this hypothesis, we investigated the effects of antioxidant on formaldehyde‐induced genotoxicity in A549 Cell Lines. Formaldehyde exposure caused induction of DNA–protein cross‐links (DPCs). Curcumin is an important antioxidant. Formaldehyde significantly increased malondialdehyde (MDA) levels, and decreased superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) activity. In addition, the activation of NF‐κB and AP‐1 were induced by formaldehyde treatment. Pretreatment with curcumin counteracted formaldehyde‐induced oxidative stress, ameliorated DPCs and attenuated activation of NF‐κB and AP‐1 in A549 Cell Lines. These results, taken together, suggest that formaldehyde induced genotoxicity through its ROS and lipid peroxidase activity and caused DPCs effects in A549 cells. Copyright © 2012 John Wiley & Sons, Ltd.