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Determination of free malonaldehyde formed in liver microsomes upon CCl 4 oxidation
Author(s) -
Ichinose T.,
Miller M. G.,
Shibamoto T.
Publication year - 1994
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550140611
Subject(s) - chemistry , microsome , lipid peroxidation , horse , methylhydrazine , chromatography , in vitro , capillary gas chromatography , gas chromatography , biochemistry , antioxidant , organic chemistry , biology , paleontology , propellant
Free malonaldehyde formed in the microsomes prepared from livers of monkey, rat, rabbit, mouse, cow, pig, dog, sheep and horse upon CCl 4 oxidation was derivatized by reaction with N ‐methylhydrazine to form 1‐methylpyrazole which was subsequently analyzed by capillary gas chromatography. Among the livers from animals tested, the monkey and rat livers produced the most malonaldehyde upon CCl 4 treatment. Horse liver showed the greatest resistance to CCl 4 oxidation. The gas chromatography method used in the present study exhibited an accurate and specific measurement of free malonaldehyde that might provide an understanding of the biochemical process of in vitro lipid peroxidation.