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Effect of tordon 2,4‐D 64/240 triethanolamine BR on the energy metabolism of rat liver mitochondria
Author(s) -
Pereira Luiz Fernando,
Campello Annibal P.,
Silveira Orieta
Publication year - 1994
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550140105
Subject(s) - mitochondrion , cytochrome c oxidase , respiratory chain , chemistry , biochemistry , nad+ kinase , oxidative phosphorylation , reductase , biophysics , enzyme , biology
Tordon herbicide, which is a mixture of 4‐amino‐3,5,6‐trichloropicolinic acid (picloram) and 2,4‐dichlorophenoxyacetic acid (2,4‐D), depresses the phosphorylation efficiency of the rat liver mitochondria, as inferred from the decrease of the respiratory control coefficient and the ADP/O ratios when NAD + ‐dependent substrates were used; NADH oxidase and NADH cytochrome c reductase were also inhibited, without any effect on the other enzymatic complexes of the respiratory chain. Tordon (66.2 nmol picloram + 270 nmol 2,4‐D mg −1 protein) affected the amplitude of swelling induced by glutamate, succinate, ( N,N,N′,N′ ‐tetramethyl‐ p ‐phenyldiamine + sodium ascorbate and ATP. These results characterize an interaction of Tordon with complex I of the respiratory chain and also a partial collapse of the proton motive force of the mitochondrial inner membrane without affecting its elasticity.