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Induction of chromosomal aberrations and sister chromatid exchange in vivo and in vitro by the insecticide cypermethrin
Author(s) -
Amer Soheir M.,
Ibrahim Aziza AbdEl Samie,
ElSherbeny Kawser M.
Publication year - 1993
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550130508
Subject(s) - cypermethrin , spleen , sister chromatids , bone marrow , sister chromatid exchange , biology , in vivo , microbiology and biotechnology , andrology , in vitro , chromosome , genetics , immunology , medicine , pesticide , gene , agronomy
The induction of chromosomal aberrations and sister chromatid exchange in vivo in mouse spleen and bone marrow as well as in vitro in cultured mouse spleen cells by the insecticide ‘Cypermethrin’ ( cistrans 1:1) was investigated. The percentage of chromsomal aberrations in the spleen and in the bone marrow as almost the same and reached its maximum 6 h following i.p. injection. The aberrations induced were chromatid and chromosome gaps, fragments and tetraploidy. The insecticide caused a significant and dose‐dependent increase in the frequency of sister chromatid exchanges (SCEs) in mouse bone‐marrow cells: it reached 11.12 ± 0.05 per cell after treatment with Cypermethrin at 300 mg kg −1 body wt. compared with 3.7 ± 0.14 per cell and 4.4 ± 0.26 per cell in the solvent and control, respectively. The percentage of viable cells in mouse spleen cell cultures reached 87.4% and 99.9% relative to the control after treatment of the cell cultures with 10 −3 and 10 −7 Cypermethrin, respectively. All the tested concentrations of Cypermethrin (0.25–400 μg ml −1 ) induced a high percentage of metaphases with chromosomal aberrations after 4 h of treatment. The mean frequency of SCEs per cell reached 15.1 ± 0.05 after treatment with Cypermethrin at 4.00 μg ml −1 compared with 8.6 ± 0.23 and 5.9 ± 0.39 in the solvent and control, respectively. The results indicate that Cypermethrin is genotoxic in mouse spleen and bone marrow as well as in cultured mouse spleen cells.