Premium
Gas chromatographic determination of vaporphase biomarkers formed from rats dosed with CCl 4
Author(s) -
Dennis K. J.,
Ichinose T.,
Miller M.,
Shibamoto T.
Publication year - 1993
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550130414
Subject(s) - chemistry , formaldehyde , chromatography , acetaldehyde , acetone , gas chromatography , thiobarbituric acid , mass spectrometry , organic chemistry , antioxidant , ethanol , lipid peroxidation
Sprague‐Dawley rats dosed with CCl 4 (3 ml kg −1 ) were placed in a glass chamber through which air was passed continuously at a rate of 60 ml min −1 . Volatile aldehydes and ketones in expired air from rats were derivatized to thiazolidines by passing the effluent gas stream through an aqueous cysteamine solution. The thiazolidine derivatives were then extracted and analyzed by gas chromatography with a nitrogen‐phosphorus detector and gas chromatography/mass spectrometry. The compounds identified were formaldehyde, acetaidehyde, acetone and formyl chloride. There were no appreciable differences in levels of formaldehyde and acetaldehyde between CCl 4 ‐dosed rats and control rats, whereas the levels of acetone in CCl 4 ‐dosed rats showed an increase compared to those in control rats. Results suggest that acetone is the major volatile carbonyl compound produced following acute doses of CCl 4 . Results of thiobarbituric acid assay on the livers from a control rat and a CCl 4 ‐dosed rat did not show any appreciable differences.