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Alterations in hepatic biochemistry of mice intoxicated with MIC, carbaryl and thiram
Author(s) -
Gupta Meenakshi,
Amma M. K. P.
Publication year - 1993
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550130108
Subject(s) - carbaryl , chemistry , thiram , cytochrome , pharmacology , microsome , cytochrome p450 , intraperitoneal injection , toxicity , hexobarbital , enzyme , alkaline phosphatase , biochemistry , toxicology , medicine , endocrinology , biology , pesticide , organic chemistry , agronomy
Abstract The effect of different doses of methyl isocyanate (MIC), carbaryl and thiram on liver microsomal mixed‐function oxygenases (MFO) was studied in adult Swiss Portan mice by intraperitoneal (i.p.) injection for different durations. The LD 50 dose of all three toxicants after 0.75 h of administration could increase cytochrome P‐450 and cytochrome b 5 contents (82–143%), and the 1/4 LD 50 of these compounds could elicit the same effect after 168 h (168–393%). The 1/4 LD 50 dose of thiram decreased the cytochrome P‐450 content below the control level (69.62%) in 0.75 h and the same dose of MIC could decrease the cytochrome P‐450 level by 40% compared to the control after 3 days of consecutive injection. The activities of drug‐metabolizing enzymes (aminopyrine demethylase—NADH and NADPH‐linked—and aniline hydroxylase) were found to increase with all three compounds in general. Marked changes in the activity of the marker enzyme glucose‐6‐phosphatase were also seen after i.p. injection if MIC, carbaryl and thiram. These findings suggested that these compounds were hepatotoxic, which could be due to their carbamylating nature.