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Copper‐induced non‐selective permeability changes in intracellularly perfused snail neurons
Author(s) -
Kiss Tibor,
Györi János,
Osipenko Oleg N.,
Maginyan Silva B.
Publication year - 1991
Publication title -
journal of applied toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.784
H-Index - 87
eISSN - 1099-1263
pISSN - 0260-437X
DOI - 10.1002/jat.2550110509
Subject(s) - helix pomatia , biophysics , permeability (electromagnetism) , snail , chemistry , copper , electrophysiology , kinetics , steady state (chemistry) , membrane , membrane potential , biochemistry , biology , neuroscience , ecology , physics , quantum mechanics , organic chemistry
The effect of extracellularly applied Cu 2+ was studied on isolated intracellularly perfused Helix pomatia neurons. It was found that the Cu 2+ ‐activated current ( I Cu ) is biphasic and composed of overlapping outward and inward components. The outward component of I Cu is the result of a blockade by Cu 2+ of the steady‐state outward Cl − current. The inward component is assumed to flow through Ca 2+ ‐activated non‐selective cationic channels. The washing‐out procedure resulted in a large inward current ( I w ), which was composed of transient and steady‐state components. It is most likely that the activation of metabolic pumps is responsible for the transient component and the steady‐state component is the result of increased neuronal membrane permeability for Cl − . Moreover, both I Cu and I w were highly Ca 2+ ‐ and temperature‐dependent processes. It is concluded that Cu 2+ application resulted in complex permeability changes in the Helix pomatia neurons .